2.3 SNP selection and detection
According to the literature, two SNPs of the IFIH1 gene were selected; rs1990760 and rs2111485 (Feizollahi et al., 2023; Jiang et al., 2019; Maiti, 2020; Muñiz-Banciella et al., 2023). SNPs were genotyped using the tetra-primer amplification refractory mutation system-polymerase-chain-reaction (T-ARMS-PCR) method as previously described (Medrano and De Oliveira, 2014). A web-based service for T-ARMS-PCR was used to design primers, which were validated using the PrimerBLAST-NCBI database. Details of primers and T-ARMS-PCR conditions are given in Table 1.